Abstract: Objective　To provide a novel method for tail vein injection in mice, aimed at improving efficiency and success rates.Methods　One hundred and twenty SPF C57BL/6J wild-type mice were randomly divided into four groups:cage cover suppression injection group, fixed lamp light injection group, conventional distal tail vein injection group, and proximal tail vein injection group (experimental group), with 30 mice in each group. Mice were restrained using a tail vein fixator and the tail was secured using a specific technique. Insulin syringes were used for injecting by proximal tail vein. The success rates of various puncture method were recorded and statistically analyzed using SPSS version 27.0.Results　Compared to the methods of restraining with cages, stabilizer with light exposure, and conventional distal tail vein injections, the proximal tail vein injection method demonstrates significantly improved success rates (P<0.05).Conclusion　This approach is accurate, efficient, and convenient, offering a new perspective for tail vein injection in mice.

Key words: mice, drug administration routes, tail vein injection, laboratory techniques and procedures

摘要： 目的　为小鼠尾静脉注射提供一种新方法,旨在提高效率与成功率。方法　选取120只SPF级C57BL/6J野 生型小鼠,随机分成4组:笼盖压制注射组、固定器灯照注射组、常规远端尾静脉注射组、近端尾静脉注射组(实验 组),每组30只。实验组采用尾静脉固定器固定小鼠并用特定手法固定尾部,使用胰岛素注射器对小鼠进行近端 尾静脉穿刺注射,记录多种穿刺方法的穿刺成功率,采用SPSS 27.0软件分析各组间的统计学差异。结果　相较于 笼盖压制法、固定器灯照法、常规远端尾静脉注射法,近端尾静脉注射法的成功率显著提高(P<0.05)。结论　本 方法操作准确、高效、便捷,为小鼠尾静脉注射操作提供了一种高效率、高成功率的新方法。

关键词: 小鼠, 给药途径, 尾静脉注射, 实验技术与操作